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Course Title[Course Code]:Genetic microtechnique (Advanced)[Gen 707]

Faculty: Agriculture
Department: Genetics
Program: Cytogenetics
Compulsory / Elective:Compulsory
Postgraduate(Doctoral-First Semester or Second Semester)
Hours:
Lecture:( 2 ) Practical / Clinical:( 2 ) Tutorial:( - )

Course Description:
Principles and applications, simple, compound, phase-contrast and fluorescent microscopes. Electron microscopy: SEM and TEM. Centrifugation Techniques: Principles, type of centrifuges, density gradient centrifugation in isolation of cells, cell organelles and biomolecules. Electromagnetic spectrum, Beer Lambert’s Law. Photometry, UV/VIS Spectrophotometry, Infrared spectroscopy, Atomic absorption spectroscopy, ESR and NMR spectroscopy. Mass spectroscopy (LC-MS, GC-MS). Fluorescent spectroscopy. Applications of different Spectroscopic techniques in Biology. Introduction and types of chromatography, paper, thin layer, gas, Gel permeation, ion-exchange, HPLC, FPLC and affinity chromatography and instrumental details of each. Applications of Chromatographic techniques in Biology.