This paper reports the pattern of ototoxicity of cisplatin on pigs using a
new technique for preparing undeclactftd bone sections by embedding the
cochlea in Methyl methacrylate (MM/U. Forty-four guinea pigs were divided
into a control group (8 animals) and 2 study groups (group I and group
II), each comprised 18 animals. The animals of group I (acute treated
group) were injected with 2 intraperitoneal (LP) injections of cisplatin (7.5
mg / kg / day) separated by interval of 5 days (total dose 15 mg / kg) i.e., a
dose largely exceeding the LD 50 dose (9.7 mg/ kg by a single I.P. injection)
and the animals of group II (chronic treated group) were injected by
a dose (1.5 mg/kg/ day) for 10 consecutive days (total dose 15 mg /kg).
Results showed that the acute treated group was affected by degenerative
changes in the organ of corn, stria vascularis, spiral ganglion cells
more than chronic treated group and the affection was more prominent at
basal turns more than middle and apical turns. Also the results showed
that the new method for preparing undecalcified specimens by embedding
in MMA had an upper hand in demonstration of fine details of delicate
structures of the cochleae more than traditional method which depends
on decalcification of bone. |
