The effect of DDB pillules on experimentally induced hepatic toxicity in rats
was • investigated in the present work. Two models of hepatic toxicity were induced,
namely, paracetamol and galactosamine toxicity. The former represents the druginduced
toxicity while the later represents the biochemical and histopathological
changes resembling those occurred in acute viral hepatitis in man.
30 male albino rats, weighting 120-150 grams, were utilized. Rats were
divided into 5 equal groups, and all were fasted overnight prior to the study. Group
1, served as untreated control group . Groups 2,3 were subjected to paracetamol
hepatic toxicity for 24 hours, by the intraperitoneai injection of 1 g/kg as a single
dose . In groups 4,5 the hepatic toxicity was induced by a single intraperitoneal
injection of 300 mg/kg D-galactosamine . Three days prior to the induction of
hepatic toxicity, rats- received three oral doses of 200 mg/kg DDB once daily in
groups 3& 5.
Blood samples were collected - by the retro orbital method. Liver function
was assessed by the determination of bilirubin concentration and both transaminases
and alkaline phosphatase activities. Rats were sacrificed by decerebration and livers
were removed for histopathological examination.
Results obtained in the present study revealed that DDB markedly protected
the liver against both paracetamol as well as galactosamine induced hepatic toxicity
as evidenced by the impro ved liver biochemical and histopathological finidings. |
