This work was done to investigate the effect of hydroxocobalamin on
nitric oxide mediated relaxation of aortic strips of rabbit. We investigate the
effect of hydroxocobalamin on rabbit aortic strips with intact endothelium and
rabbit aortic strips with denuded endothelium. In rabbit aortic rings,
hydroxocobalamin (30μM) produced concentration dependent reduction of the
relaxant action of nitrie oxide (NO) in rabbit aortic rings with or without
endothelium. Hydroxocobalarnin (30μM) had no effect in submaximal
relaxation elicited by papaverine in aortic rings. Hydroxocobalarnin (101.4.M)
produced concentration dependent reduction of the relaxant action of
acetylcholine which is mediated through the endothelium dependent nitric
oxide. A concentration of (30μM), Hydroxocobalarnin has been abolished the
responses to acetylcholine. From this study we can detect that
hydroxocobalamin reduces responses to (NO) in rabbit aortic rings by
sequesting nitric oxide so that the concentration of (NO) available for activating
soluble guanylate-cyclase enzyme relaxing the smooth muscle is reduced. In
conclusion, hydroxocobalamin may serve as an additional tool for investigating
nitric oxide mediated physiological processes. |
