Thirty albino rats weighing from 100 to 150 gm were randomly
divided into 3 groups, the first group fed standard balanced diet, the
second group fed high fat diet and the third group fed high fat diet but in
addition each rat in this group received chloroquine in a daily dose of
90mg/kg orally.
After 8 weeks of these dietary regimens, the rats were decapitated
and soleus muscle and epididymal or parametrial fat were taken from all
animals in each group. Both basal and insulin stimulated glucose uptake
was estimated in every tissue . The levels of glucose, insulin, total
cholesterol, triglycerides, high density lipoprotein (HDL) cholesterol and
low density lipoprotein (LDL) cholesterol in the serum of each rat were
also evaluated.
Results of the present work showed that persistant intake of fat
rich diet significantly reduced the basal and insulin stimulated glucose
uptake by both muscle and fat tissues. Furthermore, there were also
significant increases in the fasting serum concentrations of glucose, total
cholesterol, triglycerides and LDL . On the other hand, significant
reductions in the levels of fasting serum insulin and HDL were also
observed.
Chloroquine administration significantly abolished the inhibitory
effect of high fat diet on glucose uptake. It also induced significant
decreases in serum concentrations of glucose, total cholesterol,
triglycerides and LDL as well as significant increases in serum levels of
insulin and HDL .
In conclusion, the persistant intake of high fat diet reduces glucose
utilization and participates in hyperglycemia and dyslipidemia
Chloroquine increases muscle and fat tissue insulin responsiveness as
well as it abolishes dyslipidemia and subsequently can have favourable
effects on insulin resistance . Accordingly, chloroquine may have a
therapeutic potential in the treatment of diabetes mellitus. |
