Background and aim: The relationship between insulin resistance (IR), hypovitamine D and cerebral
infarction and its exact mechanism are not fully understood. However, oxidative stress and proinflammatory
mediators may be involved. Thus, the aim of the present study was to investigate the
effects of vitamin D on cerebral infarction, insulin resistance and inflammatory mediators in rats
received high-fructose-diet.
Material and methods: Eight four adult albino rats were divided randomly into 3 groups, normal
control group, diabetic group rats received high fructose diet for 2 months without no treatment,
diabetic rats received alphacalcidol (10pg//kg/day, orally), which was continued daily throughout the
experiment, After 2 months, fasting blood glucose level and insulin, IR was evaluated by the
homeostasis model assessment method (HOMA-IR). Some cerebrovaseular risk markers as lipid
profile (total cholesterol, IIDL-C and triglycerides), as well as inflammatory biomarker interleukin-6
and cerebral infarction size Were measured.
Results: Rats had high fructose diet showed low 1, 25 (OH)2 D, with a significant (p <0.05) increase
in fasting blood glucose level and higher HOMA-IR index, total cholesterol, low-density lipoprotein
cholesterol (LDL-C), triglyeeride and IL-6 as compared to control group. There were significant
correlations between 1, 25 (OH)2 D levels and HOMA-IR index (r=-0.47; p <0.01), I, 25 (OH)2 D,
total cholesterol (1=-0.34; p <0.01); HDL-C (r=0.54; p <0.01), LDL-C(R---0.34; P <0.01), IL-6 (r---
0.33; p <0.05). A two month oral al phacalcidol (10 ug/kg/day, orally) treatment markedly decreased
HOMA-IR index (p <0.001), LDL, triglycereides and I1-6 and significantly reduced total cholesterol
and cerebral infarction size. |
