Background: Temporal lobe epilepsy is a longer neurological disorder
in which patients suffer from spontaneous seizures. New treatments
with novel mechanisms of action are needed to help those patients
whose seizures are resistant to available drugs. In the present study, we
evaluate the possible anticonvulsant effect of ivabradine, a
hyperpolarization activated cyclic nucleotide-gated channel (HCN)
blocker in kainite model of temporal lobe epilepsy in rat. In addition,
evaluate the oxidative stress and nitric oxide alternation Material and
Methods: adult male albino rats were divided into three equal groups:
control group (CN), kainic acid (KA) induced epileptic non- treated
rats, epileptic rats treated with ivabradine (20mg/kg/day) one week
before convulsant state with KA epileptic rats. The latency to and the duration of tonic-clonic
seizures were recorded. Also, brain oxidative stress markers were measures (lipid
peroxidation, and nitric oxide). Results: of the present work revealed that convulsive state
induced by KA (10 mg/kg I.P) resulted in significant elevation (p5„0.05) of NOx (NO
metabolites, NO2" plus NO3" as indices of NO generation), lipid peroxidation
malondialdehyde in the KA—alone treated group compared with normal rats. Administration
of ivabradine (20mg/kg I.P) resulted in significant reduction (P<0.05) of duration of clonic
seizures, cortical NOx and lipid peroxidation. On the other hand, ivabradine significantly
increased latency of tonic-clonic seizures. Conclusion: The present data promising the
anticonvulsant and potent antioxidant effects of ivabradine in epileptic animals. |
