Differentiation between E. histolytica and non pathogenic species as Entamoeba
dispar, which are morphologically identical species, is essential for rapid
treatment decision, precaution of the invasive disease and public health. This
study evaluated the real time PCR for detection of intestinal amoebiasis in comparison
with microscopic examination.
Stool samples were obtained from symptomatic 40 patients from outpatient
clinic of Benha University Hospitals and Benha Educational Hospital. Twenty
suffered from dysentery and 20 cases were not suffering dysentery. In addition
10 stool samples of other parasitic infection as Giardia latiblia and Cryptospordium
parvum were examined by direct smear, iodine stained smear, formol
ether concentration technique and real time PCR for detection of E. histolytica
DNA. Formol ether concentration technique showed that 20 (40%) samples were
positive for E. histolytica but real time PCR showed 26 (52%) positivity. All
samples positive by microscopy were also positive by Pannd additional 6
PCR positive cases. There was no cross reaction with other parasites as G. latnblia
and C. parvum.
Key words: PCR, stool examination, E. histolytica, E. dispar, Giardia lamb- |
