In this work, we study the effect of certain non steroidal
antiinflammatory drugs (acetyl salicylic acid, indomethacin, naproxen,
and piroxicam) on the activity of carbonic anhydrase enzyme in vitro and
in red cell of experimental rats.
We study the effect of these non steroidal anti-inflammatory
drugs on the activity of carbonic anhydrase enzyme alone and in
association with histamine, calcium, prostaglandin E2, and acetazolamide
which is a specific inhibitor of carbonic anhydrase enzyme. The results
show that, the non steroidal anti-inflammatory drugs used here increased
the activity of carbonic anhydrase enzyme in a dose dependent manner.
Histamine and calcium added to non steroidal anti-inflammatory drugs
amplified the activating effect of non steroidal anti-inflammatory drugs
on carbonic anhydrase enzyme. Association of prostaglandin E2 or
acetazolamide (which is a specific inhibitor of carbonic anhydrase
enzyme) to non steroidal anti-inflammatory drugs, reduced their
activation on carbonic anhydrase enzyme. Indomethacin abolished the
inhibitory effect of acetazolamide on carbonic anhydrase enzyme. We
can say that the non steroidal anti-inflammatory drugs which reduce the
activity of cyclooxygenase enzyme and prostaglandin production,
increase the activity of carbonic anhydrase enzyme. These data imply
that there is an inverse relationship between carbonic anhydrase enzyme
and cyclooxygenase enzyme, that is to say carbonic anhydrase activation
being accompanied by reduction of cyclooxygenase activity, a reduction
may be achieved by the pH modifications induced by carbonic anhydrase
activation. In this way, cyclooxygenase inhibition occurs via carbonic
anhydrase with the pH variations it brings about. |
