Doxorubicin (DOX) is an anthracycline antibiotic which has a nephrotoxic
effect. Tramadol(TRAM), an analgesic, which is often used to control acute and
chronic pain. The aim of this study was designed to assess the nephrotoxicity effect of
dazorubicin (DOX) and TRAM each alone and in combination. The rats were divided
into four groups, control, DOX (4mg/kg, i.p), TRAM (54mg/lcg, i.p) and DOX&TRAM
(4n2g/kg &54ing/kg,tp,respectively), doses were administered every three days for
two weeks. At the end of the two weeks urine and blood were collected to evaluate
serum creatinine, urea, creatinine clearance, urine volume. Also ratio of kidney
weight to body weight (KW/B TV) was assessed. Kidney tissue were removed for
determination of antioxidant enzymes ('reduced glutathione (GSH), glutathione S
transferase (GST) and lipid peroxides (TBARS). Light microscopy was also done. The
activity of reduced glutathione and GST were significantly decreased, level of TBARS
was higher significantly in DOX and DOX & TRAM groups compared to control.
Serum urea ,creatinine were increased significantly in DOX & TRAM group. AB W
and FBIV were decreased significantly compared to control, also urinary flow rate
was increased and creatinine clearance was decreased in DOX & TRAM group
compared to control. On the other hand, light microscopic examination results
showed that atrophy in glomeruli decreased in its cellularity in DOX treated group
.also in sonic Bowman's capsule ,there was thickening in partial layer & narrowing in
renal tubules. TRAM treated group showed normal tubules and atrophy in glomerulL
DOX + TRAM treated group showed exaggeration in all features, vacculation and
edema in glomeruli (fig 4 -7). |
