ischemia/reperfusion (I/R) injury is considered as a major clinical problem
It induced tissue injury and apoptosis mainly via oxygen free radicals,.
Leading to the development of local and distant organ dysfunction,
Objective: This study investigates DNA damage to the heart and kidney, as
a result of intestinal UR, and possible DNA protection through the
administration of epigallocatechin-3-gallate (EGCG). EGCG was chosen to
be investigated in this model based on previous studies showed its
antioxidants, anti- fibrotic and anti-apoptotic effects. Materials and
methods: A total of 32 rats were randomly divided into four experimental
groups: sham, UR, UR pretreated with EGCG (50 mg/kg ip 30 mm before
ischemia) and EGCG treated (50 mg/kg ip). Apoptosis was assessed using
comet assay. Indices of heart and kidney damage were measured (caspase-
3,8-hydroxydeoxyguanosine and heat-shock protein-70).Results: Intestinal
UR caused heart and kidney damage as noted by significant increased DNA
parameters (DNA tailed %, DNA untailed %, DNA Tail length , Tail DNA
% and tailed moment), increased serum level of caspase-3,8-
hydroxydeoxyguanosineand and heat-shock protein-70. EGCG significantly
reduced DNA damage parameters on the comet assay in heart and kidney
homogenate, and reduced serum levels of caspase-3,8-
hydroxydeoxyguanosineand and heat-shock protein-70. Conclusion: Based
on our results, we conclude that EGCG can protect the heart and kidney
against intestinal UR injury via an anti-apoptotic mechanism. |
