The present work was performed to study the effects of
commonly used three drugs ( cefotaxime, ofloxacin and amikacin)
on some parameters of the liver and kidney functions of normal
albino rats. In addition , liver and kidney of rats were examined by
electron microscope for any histopathological changes. In this
study, sixty male normal albino rats ( 120- 180 gm each ) were
divided into 4 groups: 15 rats served as control, 15 rats treated daily
with cefotaxime (30 mg / kg) , 15 rats treated daily with ofloxacin (
10 mg / kg ) and 15 rats treated daily with amikacin ( 7.5 mg / kg ).
Cetbtax i me and amikacin were given intraperitoneally
(1.P) while ofloxacin was given orally in a freshly performed
solutions 2 hours after feeding the animals. After 14 days from
drugs administration, 10 rats from each group were used for
examination. Blood samples were collected from the retrobulbar
plexus and analysed for determination of total serum bilirubin,
serum glutamic oxalacetic transferase ( SCOT), serunylutamic
pyruvic transferase ( SGPT), serum alkaline pasphaye (ALP),
serum urea and creatinine. After obtaining the blood samples, .the
animals were killed and their livers and kidneys were removed and
examined by the electron microscope for any histopathological
changes. Five rats from each treated group were left without
treatment for 7 days. After this period, all the above investigations
were carried out. The results of this work as regards the liver
functions showed that there was a singnificant increase in total
serum bilirubin in both cefotaxime and ofloxacin treated goups, the increase induced by ofloxacin was more. In amikacin - treated
group, liver functions were nearly toward normal.
Concerning the renal functions, there was a singrtificant
increase in serum urea and creannine in amikacm and ofloxacin -
treated groups. All changes observed in the parameters measured
were found to be transient and reversible as evidenced by return of
the values to the control levels after stoppage of treatment.
Histopathological results of liver and kidney showed no significant
changes between any of the three groups which correlate with the
reversibility of biochemical results.
Therefore, it could be concluded that therapy with the above
drugs must be controlled by regular assessment of the liver and
renal function tests even in asymptomatic patients. |
