Abstract
The present study was planned to clarify the effect of replacement
therapy of estrogen and progesterone on ovariectomized rats. 49 adu/t female
albino rats each weighing 200-250 grams were utilized in this
study The animals were divided into two major groups, the first group
served as control group. it was further subdivided into three subgroups.
subgroup la comprised the non ovariectomized female rats which received
no treatment, subgroup lb. Sham operated group animals, subgroup lc: received
0.1 ml olive oil i.m once per day for two consecutive days after 4
weeks of ovariectomy and served as control for estrogen and progesterone
treated groups.
The second group was subdivided into 4 subgroups. Subgroup Ua: included
ovartectomized rats, subgroup lib: ovariectomized rats treated
with progesterone 12.5 mg /kg. Subgroup 11c: ovariectomized rats treated
with estrogen 0.38 mg/kg. Subgroup Ild: ovariectomized rats received
combined treatment of both estrogen and progesterone.
The non ovariectomized rats were sacrificed at oestrus cycle as denoted
by daily vaginal smear, while the remaining groups were sacrificed after
4 weeks of ovariectomy by 48 hours, both light and scanning electron
microscopy were performed.
Results showed that exogenous progesterone increased mucosal folding
with short bulb or rosette shaped microvilli. Estrogen resulted in epithelial
cell proliferation and keratinization. Cell separations were obvious |
