Publications of Faculty of Medicine:EFFECT OF HORMONAL REPLACEMENT THERAPY ON THE VAGINAL EPITHELIUM OF OVARIECTOMIZED RATS: Abstract

Title:
EFFECT OF HORMONAL REPLACEMENT THERAPY ON THE VAGINAL EPITHELIUM OF OVARIECTOMIZED RATS
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Abstract:

Abstract The present study was planned to clarify the effect of replacement therapy of estrogen and progesterone on ovariectomized rats. 49 adult female albino rats each weighing 200-250 grams were utilized in this study. The animals were divided into two major groups, the first group served as control group, it was further subdivided into three subgroups, subgroup la comprised the non ovariectomized female rats which received no treatment, subgroup lb, sham operated group animals, subgroup lc: received 0.1 ml olive oil Lm once per day for two consecutive days after 4 weeks of ovariectomy and served as control for estrogen and progesterone treated groups. The second group was subdivided into 4 subgroups. Subgroup lla: included ovariectomized rats, subgroup fib: ovariectomized rats treated with progesterone 12.5 mg /kg. Subgroup 11c: ovariectomized rats treated with estrogen 0.38 mg /kg. Subgroup 11d: ovariectomized rats received combined treatment of both estrogen and progesterone. The non ovariectomized rats were sacrificed at oestrus cycle as denoted by daily vaginal smear, while the remaining groups were sacrificed after 4 weeks of ovariectomy by 48 hours, both light and scanning electron microscopy were performed. Results showed that exogenous progesterone increased mucosal folding with short bulb or rosette shaped microuilli. Estrogen resulted in epithelial cell proliferation and keratinization. Cell separations were obvious