Peritoneal fluid (PF) samples were collected through laparoscopy from 60 female patients to study its
effect on in vitro early embryogenesis. Thirty of them were complaining of unexplained infertility and the
remaining 30 were fertile, requesting laparoscopic tubal sterilization and were considered as a control group.
The PF samples were subjected to (1) estimation of total protein concentration, (2) detection of different
protein bands by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), (3) qualitative
estimation of TNF-a by Western blotting, and (4) study its effect on in vitro cleavage of 2-cell mouse embryos.
The total protein concentration was significantly higher in PF of unexplained infertility group (mean 27.4
mg/ml) than control group (mean 21.07 mg/ml) (P <0.05). Nearly, the same protein bands were detected by
SDS-PACE in both groups. A peritoneal fluid band of 32 IcD appeared in 5/30 cases of the unexplained
infertility group but in non of the control group. TNF-a band was detected in nitrocellulose paper (by
Western blotting) in all cases of the unexplained infertility group as compared to 7/30 of the fertile control
group. The embryotoxicity of FT of the unexplained infertility group was significantly high (P < 0.05). The
number of embryos that reached to the blastocyst stage in 2-cell mouse embryos after |
