Ch lamydop kilo pnettmoniae
(Cpneumoniae) is a common respiratory
pathogen of human that has been
linked to variety of chronic diseases at
other body sites including atherosclerosis.
It must have a mechanism by
which it reaches these sites. The circulating
monocytes are likely the vehicles
for dissemination of C.pneumoniae
from the site of primary infection to
the site of the developing disease.
Objective: The present work aimed at
detection of viable infectious
Cpneumoniae in the blood cells of normal
blood donors (NBD), assessment
of the normal donors for
Cpneunzoniae carriage in circulating
peripheral blood mononuclear cells
(PBMNCs), and testing the ability of
Cpneumoniae infected cell to transmit
infection to host cell monolayer.
Materials and Methods: 38 peripheral
blood specimens from NBD were collected.
Sera of the patients were tested
for C.pneumoniae IgG by Enzyme
Linked Immuno-Sorbent Assay
(ELISA). PBMNCs fraction of the
blood samples were immunofluorescent-
stained for microscopic identification
of Cot:eau:office-infected cells.
Isolated white blood cells (WBC) were
assayed by in vitro culture to evaluate
infection transmission of blood cell
borne Cpneuntoniae.
Results: Using ELISA assay, 17/38
(44.7%) of NBD samples were positive
IgG specific antibodies against
C.pneumoniae. Imunofluorescence
microscopy of smears demonstrated
that 7/38(18.4%) of samples contained
intracellular C pneuntoniae. In vitro
culture demonstrated that WBCs of
9/38 (23.7%) of the NBD samples harbored
infectious C pneuntoniae, that is
clinically a potentially source of transmission.
Conclusion: C. pneumoniae is present
within peripheral blood cells of NBD,
It was present in lysates of these peripheral
WBC, and can be infectious
and readily transmitted it to host cell
monolayer. |
