Bronchial asthma and atopic dermatitis are chronic inflammatory diseases characterized by increased IgE synthesis as a result of T-cell tlysregulation in particular till that synthesize IL-4, 1L-10 which are responsible for IgE antibody response. This study aimed to asses the levels of IL-4, IL-10,1E1%1-y and IgE and their role in the pathogenesis of bronchial asthma and alopic dermatitis [and their correlations with the allergic symptoms severity!. Four groups of children were studied: group I included 10 children with atopic dermatitis, group II 15 children with mild asthma, group III 15 children with severe asthma and group IV 15 healthy control subjects. In this study I1-4, 1L-10 and IFN-y level were estimated from cultured supernatant of peripheral blood mononuclear cells PBMCs by commercial ELISA technique and the level of serum IgE was measured by ELISA. Increased level of IL-4,11-10 and IgE were demonstrated in atopic dermatitis (143.3±12.7, 116.78±14.67, 715.2±3I7.9), mild asthma (161.2±14.82, 122.33±13.88, 749.19±320.59) and severe asthmatics (201.47±79.6, 167±24.85, 2160.9±1411.7) (P<0.002 and <0.001) while the level of IFN-y decreased (71.4± 16.69, 61.2± 18.23, 42.33± 23.29, respectively) (P>0.001). It is concluded that cytokine analysis is a useful tool to asses the severity of disease, this provides a rationale for the use of IFN-y as therapeutic agent in the line of treatment |
