To evaluate whether laparoscopic approach for fallopian tissue sampling is a practicable and safe method to establish a diagnosis for asymptomatic Chlamydia trachomatis infection in infertile women and the value of PCR technique for rapid detection of such cases.
Fourty five women with primary or secondary infertility of a variable durations and all of them had normal hormonal status, proven ovulation and patent tubes by hysterosalpingography. Also, all male partners had normal spermiograms.
During laparoscopy, fallopian tissue samples were obtained from the fimbriated end of the tube and for a distance 2 cm inside the lumen by tubal cytobrush inserted through a 3rd sleeve. Also, an endocervical smear was obtained with cervical cytobrush before performing the laparoscopy. Both samples were tested for Chlamydia infection using PCR technique.
The study showed that the sampling procedure with tubal cytobrush performed in all patients without complications. Adequate samples were obtained from both tubes. One patient showed chlamydial infection only in the cervix while four patients showed combined tubal and cervical infection. Other four patients were diagnosed to have tubal infection only with C. trachomatis while 36 patients were completely free from infection. |
