Abstract
Objectives: To evaluate impact of prenatal exposure to cyclophosphamide
(CF) on morphology skeleton and viscera of rats and to evaluate
the effect of folic acid (FA) supplementation on these changes.
Materials & Methods: The study included 30 pregnant female rats
divided equally into three groups: Control group, CF group injected intravenously
by CF 7 pgm/gm body weight (BW)/day and CP+FA group
injected intravenously by CF 7 pgm/gm .8W/day and received FA 100
ug/gm/day orally. Drug therapy was given daily from the 6th to 9th
days of gestation. On the 20th day of gestation, all female rats were delivered
by labarotomy. The uterus with the two ovaries were removed
and examined for the number of corpora lutea of pregnancy and the
number of implantation and resorption sites in each uterine horn.
Crown rump length, head length and biparietal diameter were measured
and BW was recorded. Apparent external gross abnormalities were
detected. Alizarine red stained skeletons were studied for counting the
number of ribs and vertebrae and examination for any skeletal abnormalities.
Skeletal specimens were examined for scoring of ossification as
being complete, delayed or absent Hematoxylin and eosin stained skeletal
sections were examined.
Results: CF induced significant reduction of number of corpra lutes
and live fetuses compared to both control and CP+FA groups. All body
measurements were significantly smaller compared to control group
with non-significantly larger measurements in ClakFA group. BW was |
