Based on the DNA fragmentation profile in gel electrophoresis and the
histopathological and biochemical changes induced by acute toxicity of aflatoxin B1 (AFB1), this study aimed to clarify the protective effect of ascorbic
acid (A. A.) on aflatoxicosis in albino rats. Forty rats were used in this work
divided in four groups, ten in each one. The la group was served as reference group (R-gr) where the rats in this group feed on basal diet only, rats of the 2Thil group were given daily dose of ascorbic acid (A.A.) 300 mg/kg I.P. for three weeks, rats of the 3rd group were given single dose of AFBI (I mg/kg) I.P, while rats of the last group were given single dose of AFBI (lmg/kg) I.P. after pretreated with daily dose of A.A. 300mg/kg I.P. for three weeks. Our results
showed that acute aflatoxicosis induced DNA fragmentation pattern (DNA
ladder) i.e. induced apoptosis while no DNA fragmentation profils were detected in the other three groups. |
