The effect of peritoneal fluid (PF) on in vitro development of 2-cell mouse embryos and the relation between cytoldne (TNF a) level and infertility were evaluated. PF samples were collected thorough laparoscopy from 30 infertile females with endometriosis (IF-E) (test group) and another 30 fertile females without endometriosis (F-NE) (control group). PF sample was subjected to study its effect on 2-cell mouse embryos development, estimation of total protein concentration, detection of different protein bands by sodium dodycyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and qualitative and quantitative estimation of tumour necrosing factor (INF-a) by Western blotting and ELISA. The embryotoxicity of PF of IF-E group was significantly high (P<0.001), embryos reached to the blastocyst stage in 2-cell mouse embryos were 57/306 embryos in IF-E group and 280/300 embryos in FNE group. The total protein concentration was statistically high in IF-E group (P> 0.001). Analysis of PF By SDS-PAGE, showed similar protein bands and the total numbers were 10-22 bands (mean 11 bands) in IF-E group and 5-8 bands (mean 7 bands) in F-NE group |
