This study was carried outS to isolate and identify the causative bacteria of cases clinically diagnosed as mucopurulent conjunctivitis (MPC), to detect the production of IgAl protease by the isolated bacteria using enzyme linked immunosorbent assay (ELISA) and SDS- polyacrylamide gel electrophoresis (SDS-PAGE), and to find out the relationship between IgA I protease production and the sensitivity of those bacteria to different antibiotics. The most common isolated organisms were diphtheroids 66/(36.9%), follwed by Haemophilus infleunzae 60 (33.5%), Staph. aureus 43 (24%) Strept. pneumoniae
29 (16.2%), Staph. epidermidis 28 (15.6%), Strept. viridans 9 (5%) and E.coli 3(1.7%). In 67% of cases one type of organism was isolated while in 59% of cases mixed cultures were detected and
10.5% showed no bacterial growth. Among the 60 cases of H. infleunzae isolates biogroup aegyptius was the most frequent group detected (46.6%) followed by biotype 11 (28.3%) biotype IV (15%), biotype 111 (6.6%), and biotype 1(3.3%). Serotyping of H. infleunzae isolates using polyvalent capsular antisera revealed that 45 isolates were untypable and 15 isolates were typable and serotype b was the most prominent one among the typable strains and was more detected in all biotypes. |
