Abstract
Background: Cisplatin is an effective chemotherapeutic agent used
in the treatment of a wide variety of solid tumors. The major side effects
limiting its clinical use is the nephrotoxicity.
The aim of this work was to study the possible protective effects of
vitamins C and E on Cisplattn-induced nephrotaricity in adult albino
rats.
Materials and Methods: Thirty adult male rats were divided into
three equal groups: The control group, Cisplatin group and Cisplatin
plus vitamins C and E group. In the control group, the rats were injected
intraperitoneally with 2m1 of normal saline /kgB.W. once daily for 3
consecutive days. In Cisplatin group, the rats were injected intraperitoneally
with Cisplatin at dose of l Omg /kg .8. W. once daily for 3 consecutive
days. In Cisplatin plus vitamins group, the rats were injected intraperitoneally
with Cisplatin as the second group, in addition to
vitamins C and E at dose of 250mg/kg.B.W.each. The vitamins were administrated
orally with a cannula one hour prior to Cisplatin injection.
All animals were sacrificed 3 days after the last injection. The kidney
specimens were prepared for light and electron microscopies.
Results: Cisplatin produced necrosis of the epithelial lining of most
of the proximal convoluted tubules with subsequent dilatations of their
lumens. Some of these tubules contained esinophilic material .The epithelial
cells of some tubules contained many vacuoles. The Cisplatin induced
focal condensation of the connective tissue and inflammatory
cells in the interstitial spaces. Electron microscopic examMation
showed that Cisplatin produced reduction in the numbers of mitochon- |
