The present method a simple enzymatic assay which is a modification
of the methods of Habig et al. {1) and Yukihiko et al. (2) of serum
glutathione -S-transferase. This method is based upon the fact that
conjugation of 1-chloro-2,4-dinitrobenzene (CDNB) and reduced
glutathione (GSH) absorb at 340 nm more strongly than either GSH or
CDNB alone. Glutathione-S-transferase is expressed in both periportal
and centrilobular hepatocytes. Centrilobular hepatocytes are easily
damaged by hypmda and toxins. Therefore estimation of serum GSH has
been regarded as a useful test in the diagnosis of early hepatocellular
disorder. The present method of serum GSH estimation proved to be able
to handle a large number of clinical samples with short analytical period
compared with other methods. Since, serum GSH disappears from blood
faster than sGOT or sGPT, so one of its major advantages was the
evaluation of recovery phase of acute hepatic disease. |
