The present study was designed to investigate the possible involvement
of apoptosis of blood neutrophils in normal subjects, rheumatoid arthritis
(RA) and osteoarthritis (OA) patients with and without some selected
natural prouducts. This study conducted on 30 normal volunteers and 85
patients; 55 cases with RA ( active , and inactive) and 30 cases with OA of
the knee . Blood neutrophils were prepared and cultured. The cells were
incubated for 24,48 and 172 hours in tissue culture medium with and without
some selected natural products (Ascorbic acid, Quercetin and Rutin).The
percentage of neutrophil apoptosis in each culture was assessed by both
morphology and DNA fragmentation.There was significent increase of the
percentage of apoptotic cells with aging in culture mediun from normal
subjects , RA and OA patientsin RA patients , there was inhibition of
neutrophil apoptosis in comparison to normal subjects and OA patients in
whom inflammatory response is less than in RA patients. There was no
significant difference in the percentage of apoptotic cells between active and
inactive RA patients. No relationship was found between apoptotic cell
percentage and disease activity. Lastly, several observations have been
made conseming the apoptotic inducing effect of selected natrual products. |
