This study aimed to evaluate immunohistochemistry in diagnosis of toxoplasmosis in
pregnant women by comparing it with ELISA test, histopathological examination
andimmunohistochemical examination. The study was carried out on 100 pregnant ladies
aging from16 to 44 years old attending the Obstetrics and Gynecology department of
Benha University hospital, 75 patients had complicated pregnancies while, 25 cases with
normal pregnancies were taken as a control group. Immunohistochemical examination of
the D&C andplacental samples of the studied groupsrevealed that, the highest percent of
apoptosis was detected amongaborted cases.The current study shows that, the total
positive IgGamong studied groups was 41% while the total positive IgMwas 11%. The
number of seropositive cases for Toxoplasma IgG by using ELISA was; 22 (59.4%)
aborted females out of them 2(5.4%) showed mild elevation, 18 (48.6%) moderate
elevation and 2 (5.4%) marked elevation, no marked elevation in IUFD and PTL cases.
The difference between groups were statistically insignificant (P>0.05).The number of
seropositive cases for Toxoplasma IgM by using ELISA was 7 aborted females. Out of
them, 2 cases showed mild elevation, 4 cases showed moderate elevation and one case
showed marked elevation. While, 3 cases of PTL were seropositive. The difference was
statistically insignificant (P>0.05).the histopathological examination of the D&C
andplacental samples of studied groupsrevealed that, the highest number of cases having
villitis is 27 out of 37 aborted females. While, 2 females from control group have
villitisELISA IgM positive cases were 10 while cases had villitis byhistopathological
examination were 11 while by Irrununohistochemical study they were 10. The control
group showed one positive female by ELISA IgM. |
