Thirty healthy adult male guinea pigs were utilized in this study. They
were divided into six equal groups. The first was used as a control group.
Acute partcreatitis was induced in the remaining 5 groups by a sublethal
dose of the organophosphate insecticide Diazirton (DV. Animals of the
2nd, 3rd, 4th, 5th, and 6th groups were sacrificed 0.5,1,2,6 and 12 hours
respectively after DZ ingestion. Pancreatic acinar alterations occurring after
DZ intake can be arranged as follow: I) excessive stimulation of the
pancreas appears after 0.5 hour, 2) initiation of intracellnlor autolytic
changes and interstitial tissue degeneration after I hour, 3) appearance
of peripheral vacuolation may be created by extracellular enzymes after 2
hours, 4) augmentation of peripheral vacuolation and occurrence of cellular
necrosis after 6 hours. 5) disappearance of peripheral vacuoladon
with reduction in the size of the acini after 12 hours. The interstitial tissues
showed increasing width and degeneration. Conclusively, the contribution
of intracellular activation of zymogen in the pathogenesis of DZ induced
acute pancreatitis beside the other mechanism of increased
intraductal hydrostatic pressure resulting from hypersecretion became evident
Also, correlation between the ultrastructural changes in pancreatic
acini and the time passing after DZ poisoning became manifest. This finding
may pay the attention of medicolegal experts to put in their consideration
the ultrastructure study of pancreas while investigating the obscure
deaths. |
