The number of tumor associated eosinophil, Cyclin Di expression and argyrophilic nucleolar organizer region (AgNOR) and
their correlation to clinicopathological factors in colorectal carcinoma were investigated. Material and methods: forty-four cases of
colorectal carcinoma, 6 adenomenatous polypi free of cancer and 5 apparently nonnal mucosa (non-neoplastic, non-parasitic) as
control were studied. All cases were formalin fixed, paraffin embedded and subjected to immunohistochemical staining fix Cyclin l)i
using Al IC technique. Also the silver col bid technique was used fin determination of A gNOR. and (iiemsa stain to evaluate the
eosinophil counts. Results: control and adammatous colonic polypi did not express Cyclin D1 while all types of colorectal
carcinoma expressed it in different percentage, which could help in early detection of malignant transformation . Cyclin
expression was significantly higher in Dukes, C colon cancer(p<0.05) and non-survivals (p410 I). Eosinophil counts was found to be
low with poor histologic differrfiation and progression of tumors (p4).05). I Ugh eosinophil counts correlate significantly with
survivors (p4).05). AgNOR score was significantly higher with increasing cell differentiation from control to adenomatous polypi to
udenocareinomit groups and with 5-year survival (p<0.(I.5). I tut no significant correlation was found with Dukes' stage (p0.0.5). A
significant inverse relationship could be found between eosinophil counts and Cyclin Di expression in all colorectal carcinoma cases
examined (pc0.05). Conclusions: eosinophil counts and proliferative markers (Cyclin Di and AgNOR) could be valuable factors
when combined for a beau prediction of colorectal carcinoma patient's outcome. |
