Pasteurella multocida (P. multocida) infection is considered one of the highly contagious diseases causing pneumonia in bovine with devastating economic setbacks globally. Recently, inappropriate usage of antimicrobial in
treatment and control makes P. multocida resistance to the most prescribed veterinary antibiotics. The current
study aimed to detect P. multocida in apparently healthy and diseased (170) cattle and (174) buffalo in four
Egyptian governorates, defined some of epidemiological aspect, phenotypic and genotypic detection of antimicrobial resistance of P. multocida strains. The overall prevalence in examined cattle and buffalo was 21.2%. The
highest infection was in young male (41.5%) in Cairo governorate (24.5%). The antimicrobial susceptibility test
of P. multocida isolates showed high prevalence of multi-drug resistance to more than one antimicrobial group
as high resistance was recorded against Penicillin-G, Ampicillin, oxytetracycline, streptomycin and sulfamethoxazole-trimethoprim but sensitive to cefquinome. The antimicrobial resistant pattern was confirmed by detection
of four antimicrobial resistance genes (tetH, ermX, blaROB-1 and aphA1) in four phenotypically drug resistance
isolates. The four isolates revealed positive results for resistance genes by PCR assay except one isolate was negative for ermX gene. The result confirms the necessity of reliable use of antimicrobials to avoid the development
drug resistance and decrease the economic losses in animal production. |
