Background:Type 2 diabetes mellitus (T2DM), which affects millions of individuals, has
become a serious health problem.The mammalian target of rapamycin (mTOR) hyperactivation is
a negatively involved autophagy that may in fact aid in theloss of β-cell function.Resveratrol
(RSV) is a naturally occurring phytoalexin, mostly found incereals, fruits, and vegetables that
have antioxidant effects. Aim:In the current work, Wistar rats exposed to high-fat diet (HFD) and
streptozotocin (STZ)-induced T2DM were evaluated for resveratrol's protective effects on
pancreatic functions, and mTOR-mediated autophagy. Materials and Methods:Four equal
groups of forty male Wistar rats were created at random.Group 1 (G1) was maintained in typical
control circumstances and given a balanced diet;G2, G3, and G4 were fed HFD for 10 weeks
continuously; at the end of the 6th week injected I/P STZ as a single dose to induce T2DM. Three
days after diabetic induction, G3 was received RSV daily for continuously 4 weeks. G4 was
received RSV in addition to Chloroquine (CQ) as autophagy inhibitor. Results:The findings
shown that RSV reduces the pancreatic dysfunctions brought on by HFD-STZ by inhibiting the
mTOR pathway through decreasing the level of mTOR, Cas-3, and p62 and increasing the level
of LC3.RSV activate autophagy and inhibit apoptotic-mediated cell death confirmed by
transmission electron micrograph findings. Conclusion:RSV is thought to protect pancreatic cells
by inhibiting the mTOR pathway, which regulates the transition between autophagy and apoptotic
machinery in diabetic circumstances. |
