I
NCIDENCE of foodborne pathogens in meat and meat products poses significant health risks to humans and
results in financial losses for producers due to market recalls. This study aimed to isolate and identify of
pathogenic Klebsiella pneumoniae from meat and its products by using conventional culture and biochemical
characters. In addition, molecular technique was applied for detection of the isolated Klebsiella pneumoniae
using gapA gene. Overall, total of one hundred (n=100) random samples of beef products including 10 from each {minced
meat, kofta, sausage, luncheon, burger, bastrma, frankfurter, liver, chicken and shawarma} were collected from Benha markets
in Qalubia Governorate, Egypt, for bacteriological examination. The antimicrobial resistances were investigated against 7
different classes of antimicrobial groups. The results showed that incidence of isolated from meat products were 10 isolates
Klebsiella. The antimicrobial sensitivity test revealed that high sensitivity to imipenem and gentamicin and high resistance to
cefotaxime, levofloxacin, amoxicillin & clavulanic acid, erythromycin and trimethoprime & sulfonamide. Further, molecular
identification of gapA gene revealed that 5 Klebsiella pneumoniae at about 391 bp. Also, screening of 3 virulence genes (mrkD,
uge, luxS) of 5 K. pneumoniae (n=5) which responsible for biofilm production showed the positive result at (226pb, 535pb,
477pb) for the examined strains. Finally; this study concluded that meat products have significant health hazards due to bad
sanitary quality, increase the load of pathogenic Klebsiella and the presence of multidrug resistant (MDR). |
