Copper oxide nanoparticles (CuO-NPs) are employed in the pharmaceutical, cosmetic, and industrial sectors. Quercetin (QC) is one of the most abundant dietary flavonoids found in fruits and vegetables. QC has anti-apoptotic, antioxidant, and anti-inflammatory properties. In this investigation, the hepatoprotective ability of QC against CuO-NP-induced hepatotoxicity in rats was assessed. Twenty-eight mature male albino rats were divided into four equal groups. Via a stomach tube, the first group (G1) was preserved as a normal control. G2 was received QC by stomach tube daily. CuO-NPs were administered to G3 every day. Daily doses of QC and CuO-NPs were administered to G4. After the four-week experiment, liver specimens and serum samples were taken from every group of rats. The results demonstrated that QC mitigated the hepatic dysfunctions brought on by CuO-NPs through improvements in serum ALT, AST, and ALP, with a noticeable increase of total proteins and albumin levels, in addition to improving the antioxidant status as seen by elevated levels of reduced glutathione (GSH), catalase (CAT), Copper oxide nanoparticles (CuO-NPs) are employed in the pharmaceutical, cosmetic, and industrial sectors. Quercetin (QC) is one of the most abundant dietary flavonoids found in fruits and vegetables. QC has anti-apoptotic, antioxidant, and anti-inflammatory properties. In this investigation, the hepatoprotective ability of QC against CuO-NP-induced hepatotoxicity in rats was assessed. Twenty-eight mature male albino rats were divided into four equal groups. Via a stomach tube, the first group (G1) was preserved as a normal control. G2 was received QC by stomach tube daily. CuO-NPs were administered to G3 every day. Daily doses of QC and CuO-NPs were administered to G4. After the four-week experiment, liver specimens and serum samples were taken from every group of rats. The results demonstrated that QC mitigated the hepatic dysfunctions brought on by CuO-NPs through improvements in serum ALT, AST, and ALP, with a noticeable increase of total proteins and albumin levels, in addition to improving the antioxidant status as seen by elevated levels of reduced glutathione (GSH), catalase (CAT), and lowered levels of malondialdehyde (MDA). Furthermore, phosphoinositide 3-kinase (PI3K) and AKT (protein kinase B) genes had their mRNA levels highly up-regulated, whereas those of nuclear factor kappa B (NFκB) and signal transducer and activator of transcription (STAT-3) genes were dramatically down-regulated. maintained liver tissue architecture, decreased immunoreactivity against caspase-3 (Cas-3). These results imply that QC might enhance the hepatoprotective benefits against oxidative stress caused by Additionally, QC maintained liver tissue architecture, decreased immunoreactivity against caspase-3 (Cas-3). These results imply that QC might enhance the hepatoprotective benefits against oxidative stress caused by CuO-NPs toxicity. |
