Background: Sepsis represents a critical condition characterized by organ dysfunction
resulting from exaggerated immune response to infection. MiRNA-146a is vital
mediators in innate immune and inflammatory responses and its association with proinflammatory cytokines production, lipopolysaccharide (LPS)-mediated. TREM-1 is
defined as an innate immunity receptor expressed on myeloid cells as monocytes and
neutrophils involved in their activation and release of cytokine. Objectives: To study
microRNA-146a expression and sTREM-1 serum levels in patients with sepsis compered
to control healthy group and assess their diagnostic and prognostic value.
Methodology: This study was carried out on 90 participants: 60 septic patients from
ICU Department, Benha University Hospital on the basis of blood culture (positive
microbiological culture results) and National Early Warning Score (NEWS), 30 age and
sex matched healthy controls. Five ml of venous blood was collected in sterile test tube
with no anticoagulants from all participants under complete aseptic condition. Serum
was separated and divided in to 2 parts for Real time PCR for measurement miRNA 146-
a expression and assess sTREM-1 levels by ELISA. Results: miRNA 146a gene
expression increased significantly in the sepsis group compared to the control group
(p |
