Garlic cloves belongs to the genus Allium and family liliaceae, is one of the more commonly used health supplements in the world. It is a medical plant known since a long time for its therapeutic benefits. Many attempts were carried out to elucidate its importance since it contains natural antioxidant enzymes, i.e. catalase (CAT) and peroxidase (POD). These natural enzymes were extracted from garlic cloves and their activities and kinetic characterization were investigated. The results indicated that the activity, protein content and specific activity of catalase and peroxidase were 2.05, 204.4 Units /ml, 4.2, 5.11mg/ml and 0.448, 40Units/mg protein, respectively. The optimum pH and temperature of the enzymes under investigation were 7.0, 5.5 and 40ºC, 50ºC, respectively. The Km and Vmax for catalase and peroxidase enzymes were equaled to 0.532, 2.70 ml/100ml and 1.56, 0.60 Units/ml/min, respectively. |
