Type 2 diabetes mellitus is a common and increasingly prevalent disease and is thus a major public health concern worldwide. The International Diabetes Federation estimates that there are approximately 387 million people diagnosed with diabetes across the globe (Santos-Longhurst A& Krucik G, 2014).
Oxidative stress plays a pivotal role in cellular injury from hyperglycemia. High glucose level can stimulate free radical production. Weak defense system of the body becomes unable to counteract the enhanced ROS generation and as a result condition of imbalance between ROS and their protection occurs which leads to domination of the condition of oxidative stress (Pandey KBet al ., 2010).
The prostaglandin, 8-iso-prostaglandin F2α (8-iso-PGF2α), is an isoprostane that is produced by the non-enzymatic peroxidation of arachidonic acid in membrane phospholipids(Basu S. ,2010).
Measurement of 8-iso-PGF2α is a reliable tool for the identification of subjects with enhanced rates of lipid peroxidation. Enhanced formation of 8-iso-PGF2α has been reported in association with several cardiovascular risk factors, as well as atherosclerosis and DM (Wang B et al., 2006).
The aim of this study is To detect relations between glucose variability & oxidative stress marker (8-iso-PGF2α) in patient with type 2 diabetes mellitus.
Patients:
This prospective study was conducted on 85 patients ( 45 patients with type 2 Diabetes mellitus & 40 healthy volunteers of matched age, sex, physical activity and BMI as a control group).
Methods
1)Full history with paying attention to age, sex, smoking,diabetic duration, diabetes therapy, complications, concomitant therapy and other diseases .
2) Laboratory Investigation including:-
A- Complete blood picture
B-Liver and kidney function tests.
C- Fasting Blood Glucose Level , blood glucose monitoring before & after meals & before bed time will be measured for two successive days per week for 3 months and HbA1c.
D-Serum 8-iso-PGF2α by ELISA.
Results
T2DM group had significantly higher BMI when compared to control group with P value less than 0.001.
T2DM group showed significantly higher creatinine and urea when compared to control group with P value 0.001& |
