: Objective: the aim of this study is to assess the circulating levels of inflammation-associated miR-155 and endothelial-enriched miR-126 to determine their regulation in children with ESRD, expecting to be able to provide new insights into the development of ESRD. Subjects and methods: in this study we investigated 50 cases which were divided into two groups as patients group: include 30 ESRD children who are receiving maintenance hemodialysis and control group: include 20 healthy children without any evidence of CKD or inflammatory disorders. Results: the expression of miR-155 and miR-126 were significantly reduced in ESRD patients. Also, but did not significantly differ between pre-HD and post-RD with slight increase in pre-HD (where miRNA-126 level quantity mean ± SD pre-HD 12.68 ± 0.58 and in post-HD 11.67 ± 1.86 compared to 15.33 ± 0.51 in control group, while miRNA-155 level quantity mean ± SD in pre-HD 13.77 ± 0.90 and in post-HD 13.19 ± 0.60 compared to 16.44 ± 0.02 in control group). Nevertheless further studies are required for the validation and extension of these results. miRNA-126 correlated significantly and positively with sex (being higher in males), while no correlation between miRNA-155 and sex. Circulating miR-155 and miR-126 levels correlated positively with eGFR, hemoglobin and CRP, while they correlated negatively with creatinine. Circulating miR-155 correlated significantly and negatively with calcium, while circulating miR-155 correlated significantly and negatively with phosphate. Conclusion: the expression of miR-155 and miR-126 where significantly reduced in ESRD patients, but did not significantly differ between pre-HD and post-HD. Our data suggested that miR-155 and miR-126 might be useful predictive tools in ESRD. The reduction of circulating miR-126 and miR-155 might be accompanied by a series of clinical symptoms.
[Abd Elhamid Elhamshary, Rabab Fawzy Salem Bayoumy, and Mohammed Elsayed Abd Elnaby Nawaya.
Circulating levels of inflammation-associated miR-155 and endothelial-enriched miR-126 in patients with end stage renal disease on regular hemodialysis. J AM Sci 2015;11(6):165-169]. (ISSN: 1545-1003).
http://www.jofamericanscience.org.19
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