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Prof. Abeer Abdelhameed Almahlawy :: Publications:

Title:
Comparative Light and Electron Microscopic Study on the Therapeutic Efficacy of Adipose Derived Stem Cells Versus Exosomes for Experimentally Induced Acute Corneal Injuries in Rats.
Authors: Abeer M El-Mahalaway, Nahla El-Eraky El-Azab, Mohamed Abdrabbo, Omar M Said, Dina Sabry and Mohamed M El Sibaie
Year: 2018
Keywords: Alkali burn; Cornea; Exosomes; Substantia propria;Keratocytes
Journal: Journal of Stem Cell Research & Therapy
Volume: 8
Issue: 6
Pages: 1-12.
Publisher: Longdom publisher
Local/International: International
Paper Link:
Full paper Not Available
Supplementary materials Not Available
Abstract:

Background: Corneal alkali injuries are considered a common ophthalmologic emergency with many serious complications. Adipose Tissue-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells derived Exosomes (MSCs-EX) are promising approaches in regenerative therapies. Objective: The aim of this study is to evaluate the efficacy of ADSCs versus MSCs-EX on experimentally alkali induced corneal injuries in rats. Materials and Methods: 40 adult male albino rats were divided into four groups, that is, Group I: control rats,Group II: alkali burn rats, Group III: ADSCs treated rats after corneal alkali burn, Group IV: MSCs-EX treated rats after corneal alkali burn. Cornea specimens were taken and processed for histological examination. Results: Group II showed obvious changes such as erosion with focal loss of some superficial epithelial cells, while the basal and intermediate epithelial cell layers had vacuolated cytoplasm with pyknotic nuclei. The substantia propria contained irregularly arranged collagen fibers with wide spacing, keratocytes, and congested blood vessels. Descemet's membrane appeared thin and degenerated with focal loss of some endothelial cells. Ultrastructural examination showed degenerated apical squamous cells with loss of microvilli on their free surface. The basal cells had irregular contour with widening of intracellular space between cells with multiple cytoplasmic vacuoles. The substantia propria showed disorganized collagen fibers with degenerated keratocytes and mononuclear cellular infiltration. Groups III and IV showed improvement of the histological and electron microscopic changes described in group II. Conclusion: ADSCs and MSCs-EX can improve corneal alkali burn injuries and prevent their complications. Exosomes are a more promising therapeutic approach.

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