Background: Bacterial vaginosis (BV) is the most prevalent form of vaginal disturbance in women of childbearing age. The aim of this study was: Diagnose of BV in the studied women and determining the associated risk factors, isolation and identification of anaerobic bacteria in diagnosed women.
Materials and Methods:This study was conducted on 150 women at their reproductive age attending The Obstetrics and Gynecology Department of Benha University Hospital as out-patients and in-patients with age range 19-40 years after taking their consents. Two high vaginal swabs were taken from each woman. One swab was placed in a tube of sterile cooked meat broth medium for anaerobic culture, the other was placed in a tube of normal saline for wet mount and Gram staining.
Results: BV was diagnosed by clinical composite criteria of Amsel and by Gram staining. Isolation of anaerobic bacteria was done on Robertson cooked meat medium followed by subculture on the general selective blood agar for anaerobes and then different selective blood agars for different anaerobes. Further identification to the species level was done using Rap ID ANA II system. BV was diagnosed in 62 women (41.3%) out of 150. Out of 62 women diagnosed as BV, anaerobes were isolated from 44 (71%), from them77 anaerobic strains were isolated. Out of which 46(60%) were Gram positive and 31(40%) were Gram negative. There was a high significant statistical correlation between isolation of anaerobes and complications of pregnancy (p |
