Out of 50 samples were taken from each carcass of camel, cattle, sheep and goat from Kalyobia province abattoirs, the incidence rates of enterohaemorragic Escherichia coli O157: H7 strain were 6, 8, 11 and 9 with the percentage of 12%, 16%, 22% and 18% respectively. In the same time out of 50 samples were taken from diarrheic human in the same localities of animals, the incidence rate of enterohaemorragic Escherichia coli O157: H7 strain was 22 with the percentage of 44%. Isolates were tested for susceptibility to 6 antimicrobial drugs by discs (AMP, ampicillin; NAL, nalidixic acid; CIP, ciprofloxacin; CHL, chloramphenicol; TET, tetracyclin; TRI, trimethoprim) diffusion and agar dilution methods in order to determine minimum inhibitory concentrations (MIC). Multiplex PCR amplification was used to detect genes conferring resistance to: ampicillin (TEM and SHV beta-lactamase), chloramphenicol (catI, catII, catIII and cml), tetracycline (tetA, tetB, tetC, tetD, tet E and tetG), and trimethoprim (dfrA1, dfrA9, dfrA12, dfrA13, dfr7, and dfr17). |
