A total of 360 cattle and 360 buffaloe carcasses of different ages and sexes were examined at Kalyobia abattoirs from January to December, 2011. The obtained results indicated that the prevalence of Fasciola species in the slaughtered cattle (3.67%) was lower than that in slaughtered buffalo (5.56%). Generally, females (6.67% & 11.67%) were more susceptible to infection with fasciolosis than males (2.08% & 2.5%) among slaughtered cattle and buffalo, respectively. However, The Histopathological examination of the liver infested with fasciola showed newly formed bile ductules with inflammatory cells infiltration and fibrosis associated with hyperplasia in the lining epithelium with polyps formation as well as the portal area showed severe fibrosis with inflammatory cells infiltration. Regarding PCR technique, the using of Eael restriction endonuclease enzyme as a genetic marker for F.hepatica is greatly effective when the enzyme uniquely fragmented the SrRNA gene into two bands without digesting the gene of F.gigantica. Out of 200 stool samples collected from human of different ages and sexes at Kalyobia province, 4%were positive for Fasciola eggs. Thus, children between one and fifteen years old represent the highest infection (5.88%) than individuals between sixteen and thirteen (4%).One the other hand, the lowest infection was observed in individuals between thirty one and forty five (1.75%). |
