For sero-biochemical study on Brucella abortus affected dairy cattle and human contacts, a total of 70 blood samples were collected from dairy cattle in different localities in Kaliobia governorate and 30 blood samples from humans in contact with dairy cattle (6 veterinarians, 12 animal attendants and 12 farmers). The collected sera were examined by rapid slide and tube agglutination tests against Br. abortus antigen. The serological investigation revealed that Br. abortus antibodies were present in 6 (8.57%) of dairy cattle and 3 (10%) human contacts.
Biochemical estimation, in the diseased cow and men compared with the healthy group, revealed a significant (P < 0.05) increase in the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (AP). There was a non significant increase in the total and direct bilirubin, while there was a highly significant (P < 0.01) decrease in total protein and albumin and significant (P < 0.05) increase in the serum globulin in diseased cows as well as a significant (P < 0.05) decrease in the total protein, a highly significant (P < 0.01) decrease in the albumin and a significant (P < 0.05) increase in the globulin in patients.
Analysis of some minerals, in the diseased cows showed a highly significant decrease in the calcium and inorganic phosphorus and a significant decrease in the magnesium and sodium levels, besides non-significant variations in the potassium. Moreover, there was significant decrease in the above minerals in the patients when compared with healthy one. However there were a non significant changes in thyroid hormone profile.
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