ABSTRACT
The first days of life represent a critical period for the new born, the present study aimed to investigate tissue antioxidant of rats puppies during the whole suckling period in response to maternal prenatal injection with Vit. E + Selenium. Twenty female albino rats at breeding age were divided into two groups: 1- control group, comprised 10 animals maintained on basal diet and not supplied by any additives. 2- Experimental group consists of 10 animals maintained on basal diet and injected with vitamin E + Selenium at a dose of 20 mg/kg.b.wt. and 150 µg/kg.b.wt., respectively, through I.M. weekly for 3 successive weeks. Both groups bred and the injection were repeated during pegnancy period in the experimental one. After labor, rat puppies were killed at intervals of one, 4, 9, 14 and 21st days whereas dams were scarified at 21st. Liver, heart and lung tissues of puppies and their dams were used for determination of glatothione peroxidase (GSHpx) Cu/Zn superoxide dismutase (SOD) and Catalase (CAT) activities in addition to reduced gluthione (GSH) concentration. The obtained data revealed significant increase in hepatic GSHpx, Cu/Zn SOD activities and GSH concentration in addition to lung Cu/Zn SOD of experimental group of dams compared to control group. Concerning rat puppies, there were significant increase in GSHpx, Cu/Zn SOD activities in heart, lung and liver of puppies obtained from injected dams at one day followed by high significant increase at 4th day that increase become non significant at 9, 14 and 21st day compared to puppies obtained from control group as well as GSH concentration showed significant increase in liver at one day and in heart, lung and liver at 4th day. These results may attributed to the transfer of selenium from placenta to newly borned and through colostrums similar to vitamin E that transferred via mammary glands.
Key Words: Vit. E, selenium, prentatal, tissues antioxidants, puppies.
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