Biochemical Effect Of Melatonin Administration On Oxidative Stress Caused By Aging In Male Rats
Afaf. D. Abd El- Magied and Hossam F. Attia
Dept. of Biochemistry & Dept. of Histology & Cytology. Fac. o Vet. Med. (Moshtohor) Benha University
ABSTRACT
Nowadays, early aging represents a big problem among people due to physical and psychic strains together with industrial and environmental impurities. The present study was undertaken to elucidate the effect of aging as well as the possible effect of melatonin administration on the oxidative stress caused by aging. Cu/Zn superoxide dismutase (Cu/Zn SOD), glutathione peroxidase (GSHPX) and Catalase (Cat) activities in addition to reduced glutathione (GSH) concentration and lipid peroxidation (malondialdehyde) level were determined in the heart , lung and kidney of all rats groups. Our results revealed that, aging induce significant decrease in the heart, lung and kidney Cu/Zn SOD, GSHPX as well as cardiac catalase activities when compared with young control group. While melatonin returned their level near to the level of control young rats. Melatonin also reversed the reduction in cardiac and renal GSH concentration caused by aging. Malondialdehyde (MDA) level in the heart, lung and kidney tissues of aged male rats showed significant increase when compared with young control group. Melatonin ameleorate MDA level near to that of control young rats. These results were confirmed by histological examination which revealed myocardial changes, destruction of the alveoli in the lung and degranulation and desquamation of the renal tubules epithelium of the kidney of aged group. These histological changes were alleviated in the melatonin administrated group. We concluded that melatonin could be successfully used in reduction of oxidative stress caused by aging in the heart, lung and kidney tissues of male rat.
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