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Dr. Ahlam Farag Elsadek Moharm :: Publications:

Title:
A novel nano magnetic beads dot ELISA immunoassay and its application in the detection of Giardia lamblia coproantigen
Authors: Nagwa S. M. Aly1, Ibrahim R. Bayoumi 2, Rabab F. Selem, Manal I. Kardoush1*, Gehan A. Rashed1 and Ahlam Moharam 1
Year: 2018
Keywords: Giardia lamblia, Dot ELISA, Nano technology
Journal: Not Available
Volume: Not Available
Issue: Not Available
Pages: Not Available
Publisher: Not Available
Local/International: International
Paper Link: Not Available
Full paper Ahlam Farag Elsadek Moharm_paper.docx
Supplementary materials Not Available
Abstract:

Background: The traditional basis of diagnosis is identification of Giardia lamblia trophozoites or cysts in the stool of infected patients. Recently the advent of more objective techniques like antigen detection methods has led to an increase in their use versus those that rely on subjective microscopic examination of fecal specimens for Giardia cysts may facilitate diagnosis of G. lamblia in stool specimens. Methods: Purified G. lamblia cysts antigen was prepared by two-phase sucrose gradient technique. Polyclonal antibody against purified G. lamblia cysts antigen was prepared and labelling with horseradish peroxidase and Nano Magnetic Beads (NMB) to be used as detecting antibody. A total of 72 stool samples, 32 samples positive for giardiasis, 20 samples positive for other parasitic infections in addition to 20 negative samples were examined using dot ELISA and NMB dot-ELISA. Results: The sensitivity of the traditional dot-ELISA was 81.3 % and it increased by using the NMB-dot-ELISA to be 96.9% in stool samples. Specificity of both techniques was 97.5%. The present study concludes that diagnosis of G. lamblia by NMB-Dot-ELISA technique is sensitive, specific, rapid and easy to perform and interpret. In this study, using the nano-magnetic beads increased the sensitivity of the applied technique.

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