The objective of the present investigation was conducted to characterize physical, chemical and functional properties as well as amino acids composition of defatted lupin (Lupinus sp.) from control and mutant varieties grown in the Egyptian Atomic Energy Authority which produced by radiation mutation breeding program. Also, the functional properties of these samples were determined. The results illustrated that a highly significant differences between genotypes in plant height, stem diameter, seed yield and weight than the local two variety. In addition, radiation mutation of lupin seeds showed slight increased in protein content as the main constituent of lupin seeds, as well as total oil percentages in some mutant of these seeds in return of decreasing in total carbohydrate. Furthermore, radiation mutation had detectable effects on the total amino acids contents of lupin seeds meal which had a higher percentages on essential amino acids (E.A.A) and nonessential amino acids (N.E.A.A) and mut.3 was the highest values on E.A.A and N.E.A.A as compared to the control one. On the other hand, radiation mutation improved the protein functional properties of some mutant of lupin meal flour than the other mutant samples as compared to local commercial varieties.