This study included 45 patients classified into 4 groups; Group I: 14 patients with gastritis, Group 11:10 patients with gastric ulcer, Group Ill: 15 patients with duodenal ulcer, and Group
IV: 6 patients with gastric cancer. Antral biopsies were taken from all patient for isolation of Helicobacter pylori (H. pylon) and for separation of its toxin. The following celiUnes were used to demonstrate the cytopathic effect (CPE) of separated toxin. African green monkey kidney (Vero), Baby hamster kidney (BHK-21), human lung carcinoma (LLC-MK2), and Humen epithelial (HEP-2). H.pylori was isolated from 27 patients (60%) and it was nonsignificantly higher in males (66. 67%) than females (46. 67%), The isolated H. pylori was nonsignificantly higher in smokers (68.75%) than nonsmokers (38.4%) in all groups except in the group IV where it was significantly higher in smokers. CPE of H. pylori toxin was highly significant on Vero cells than other cell lines used as it reached a high dilution titre of toxin (1116) in 13 isolated strains (48.15%). No significant difference was detected as regards to CPE of toxin in different dilutions on the different cell lines used in different groups. There was a significant correlation between CPE of H. pylori toxin on both BHK-21 and LLC-MK2 cell lines. H. pylori toxin could be detected by electrophoresis as a band with a molecular weight ranged between 66-97 KDa. Conclusion: H. pylori has a role in the pathogenesis of gastroduodenal diseases (gastritis, gastric ulcer, duodenal ulcer, and gastric cancer), and the Vero cell line was the most sensitive cell line for detection of CPE of its toxin. |
