Spl/lUlIl or hWllchoo/l'cojar 11II'(18C (BAL) SIH'cilJlcIIS ji"()/11 47 1'00iellls (.W 11/(/11'.1' (IIld 17 fellwlcs) I\'illt aClive newly diagnosed pulll/onary tuberculosis. 11'110 Iliere 1I01 rcccivillg allY olllilu/Jcrculous drugs before Ihe sllidy. were onalysed by Ziehl-Neclsen (ZN.). Rho(icll1line-Aurwllille (R .A.) Staining. MiddleBrook (MB.). Lowenslein-Jensen (L.l.) CulTres tlnd peR. MuraTiollS conferring 111l11Tidrug resis((Jnce were deTecTed by single-slrand conformaTion polym"/)hisl1I (SSep) aliaIys is. OIiT ofIhe 47 spuTlim spec"
imel1s. 41 cases were found TO be peR posiTive for mycobaCTerium Tuberculosis by amplificaTioll of 164 base pair (bp) or 123 bp DNA sequences from Ihe 65 K da (Kilo Dallon) heaT shock proTein gene conwined ill Ihe 15986 insenioll sequence. respeclively. The remailling 6 cases were found negative by pe R. \'hi('1t 1)I"()I'cr/ I!.\' d({/C'rclIl idellfi{icOlion IIIClllOds !O he lIIycol!a("/crill/l1 olher Flwn III/Jerclilosis (MOTT). Alllhe 47 cases lI'ere posilive by direcl SI//cor and/or cullllre (~r Ihc SpUlU/11 in 37 cases alld oI BAL il7 10 cases. Of Ihe PCR-I){)silive 1)(Iliellls. R. 10. 14 alld In pOliellls were lIegarive by M.B. cIIIlurillg. R.A. staillillg. Z,N. sWillillg (/1/(1 L.l. ("It/lurillg re.·/)cclil'e1y. 0111 (~( fllc 41 peR posilive pariclIIs. 20 pnriclIlS (48.8%) had orgonis/lls .,ensilh ·c :r (III dmgs leslcd. ",!tile 21 pariellls (50.2%) Iwd orgalliSl1ls re.l'i.l'/{./111 10 olle or l7Iorc of lite (/l1liIU/)CI'ClllolI:. dmgs. MIIWlioll.l' ill kal C gellc cO/~(crrillg reSiSl(lllCe /() isollia:id were fOllnd ill 14 0111 (~( 41 specimens (34.2 %) (d'II,flich. 4 Iwd also alleraliolls ill Ihe il7h A gene Irhi('h cO/(fer resis{(Il7ce 10 borh is()lIio~id alld eilhiollol1lide. MUlariolls ill Ihe illli A gelle Irere delecled in 70111 oj"41 specilllells (/7.1 (!c). (~( witicll 3 hod I/O cO//('ollliwllf kOl C gelle. Mutaliolls ill Ihe IpO B gene. cO/lt'Nrillg rcsi.IIOIIC(, 10 r((olllpicill i'cu' dClce/cd ill 7 0/(/ (~r41 specimclIs (17./%). of whicit 5 had also mllla/io"s ill II,C km C 8cllc. Tllcrcfore. 1I11II(/lio1lS cO/~rerrillg nfal/lfJicill rcsiSlOlICC appeared 10 he associated lI'ilh Ihose conferring isolliazid resis/{/nce. MuraTions ill Ihe rpsL gene. COIIferring resistance 10 slrepTomycin were delecled ill 8 oUT of41 specimens (19.5%). This work is a preliminary inveSTigaTion alld opens up flew fieldforfurther sludies 10 lIlodify The chemical STrUCTure ofThe ·111051 importanl effeclive anTiTUberculolls drugs il7 order 10 be flOI affecled by gene mUTaTioll and Thus reducing
Ihe incidence ofillilial drug resi.crance. |
