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Prof. Ahmed Ali Abd El Maksoud El Hosary :: Publications:

Title:
Genetic diversity among wheat genotypes using RAPD markers and its implication on genetic variability of diallel crosses
Authors: A.A.El-Hosary; M. El. M. El-Badawy; S.A.S Mehasen; A.A.A. El-Hosary, T.A. El-Akkad and A. El-Fahdawy
Year: 2019
Keywords: combining ability, drought stress, GCA, RAPD marker, SCA, Wheat.
Journal: Bioscience Research
Volume: 16
Issue: 2
Pages: 1258-1266
Publisher: Bioscience Research
Local/International: International
Paper Link:
Full paper Ahmed Ali Abd El Maksoud El Hosary_1258-1266-16(2)2019BR19-156.pdf
Supplementary materials Ahmed Ali Abd El Maksoud El Hosary_1258-1266-16(2)2019BR19-156.pdf
Abstract:

Hybrids involving diverse parental genotypes generally produce high specific combining ability effects and hybrid vigor. Therefore, the choice of the suitable breeding program depends on the inheritance of the quantitative traits. This research aims at detecting the extent of the genetic diversity (GD) among 8 wheat parental genotypes, and its implication on F1 crosses for biological and grain yield plant-1. Thus the 8 parents were screened using ten RAPD primers. These primers produced 89 fragments, 58 of them were polymorphic. A GD value as high as 0.474 was found just one cross (P1xP4). Half diallel mating, design involving 8 parents, was made to initiate a set of 28 F1 crosses. Parents and their crosses were tested under normal and drought stress conditions for biological and grain yield per plant. Genotypes, general (GCA) and specific (SCA) combining ability were significant for the two traits. The non-additive gene action is substantial in controlling these traits. Five crosses showed all showed relatively high mean performance and SCA effects for both traits in both and across normal and drought environment. For biological and per plant grain yield, Pearson’s correlation coefficients with GD were 0.34 and 0.69. Hence, RAPD marker can be used as a tool for determining the extent of genetic diversity among wheat genotypes and to precisely predict the yield performance value for F1 hybrids.

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