| You are in:Home/Publications/Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques | |
Dr. Ahmed Reda Mohamed Hassan El-Khawagah :: Publications: |
|
| Title: | Evaluation of In Vitro Capacitation of Buffalo Frozen/Thawed Sperm by Different Techniques |
| Authors: | B. Gasparrini · A.R. Elkhawagah · V. Longobardi · G.A. Sosa · G. Bifulco · M.E.A. Abouelroos · A.E. el-ghafar · G. Campanile · |
| Year: | 2015 |
| Keywords: | Buffalo Frozen Semen Capacitation Trypan Blue/Giemsa Chlortetracycline |
| Journal: | journal of reproduction and infertility |
| Volume: | 4 |
| Issue: | 3 |
| Pages: | 19-28 |
| Publisher: | Not Available |
| Local/International: | International |
| Paper Link: | |
| Full paper | Ahmed Reda Mohamed Hassan El-Khawaga_evaluation of invitro capacitation of buffalo frozen sperm by different techniques.pdf |
| Supplementary materials | Not Available |
| Abstract: |
This study aimed to determine the most reliable method to evaluate capacitation of buffalo frozen/thawed sperm. Frozen/thawed sperm were incubated in TALP medium in absence of capacitating agents (control) and in presence of 10 μg/ml heparin for 2 and 4 hrs. Capacitation was assessed by Trypan blue/Giemsa after lysophosphatidylecholine (LPC) exposure, chlortetracycline (CTC) fluorescence assay and immune-localization of tyrosine phosphorylated protein. Furthermore, we evaluated the effect of heparin on penetration, cleavage rates and kinetics of embryo development after heterologous IVF. The percentage of LPC-induced acrosome reacted (AR)-sperm increased (P |
