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Dr. Ahmed Reda Mohamed Hassan El-Khawagah :: Publications: |
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| Title: | Effect of buffalo bull breeds on developmental competence and vitrification of invitro
produced embryos |
| Authors: | Walid T.M. Soliman1, Al-Shimaa Al-H.H. El-Naby2, Karima Gh.M. Mahmoud3, Ahmed R.M. El-Khawagah2, Mohamed M.M. Kandiel2, Mahmoud E.A. Abouel-Roos2, Alaa E.Abdel-Ghaffar2, AbdEl Salam I. El Azab2 |
| Year: | 2018 |
| Keywords: | In vitro fertilization Buffalo breed Embryo production Vitrification |
| Journal: | Asian Pacific Journal of Reproduction |
| Volume: | 7 |
| Issue: | 6 |
| Pages: | 270-273 |
| Publisher: | APJR |
| Local/International: | International |
| Paper Link: | |
| Full paper | Ahmed Reda Mohamed Hassan El-Khawaga_AsianPacJReprod_2018_7_6_270_246346.pdf |
| Supplementary materials | Not Available |
| Abstract: |
Objective: To assess effect of buffalo bull breed on the development and cryotolerence of the in vitro produced embryos. Methods: Three types of frozen semen were adopted; Egyptian, Italian and cross-bred (Egyptian-Italian) breeds were used for in-vitro fertilization and vitrification of their embryos. Oocytes were collected from buffalo ovaries and matured in vitro for 24 h, then they were fertilized using the three semen breeds. The produced embryos of morula and blastocysts were vitrified using ethylene glycol and dimethyl sulfoxide then evaluated for their viability after warming. Results: The cleavage and blastocysts rates significantly declined in oocytes fertilized by Egyptian (P |
